Transcendis biotechnology improves naked mRNA transfection of mouse skin, skeletal muscle or tumor.
Naked uncapped mRNA possesses a peptide tag-binding RNA aptamer at its 5’end.
A transfection peptide possesses this tag at its C-terminal end.
Naked mRNA and the transfection peptide are mixed and allowed to bind to each other at room temperature for a few minutes.
A single copy of the transfection peptide non-covalently and strongly binds to mRNA.
The injection of such transfection peptide/mRNA system to mouse skin leads to a 9.3-fold enhancement of protein expression compared to mRNA alone.
Thus, Transcendis greatly improves the uptake of naked mRNA by skin cells.