The stability of an mRNA depends in part on the stability of a polyA at its 3’end.

Mathusalem is about the non-chemical modification of polyA, in order to render it more resistant to RNases.

It allows the production of an mRNA having a longer half-life in transfected cells.

It is also possible to reduce the size of polyA to less than 100 residues.

In a plasmid DNA, such shorter polyA/polyT is not truncated over time in E. coli.

This allows the production of mRNA having a polyA of constant size.