The first aptamer Messenger Biopharma has identified has been a cell-penetrating RNA aptamer selected on C2C12 cells.
This aptamer has been inserted in the 5’UTR of our proprietary uncapped mRNA.
The modified luciferase mRNA has been injected to skeletal muscle of living mice.
The transfection of this mRNA led to a 2.6-fold enhancement of luciferase expression in muscle compared to a similar luciferase mRNA devoid of aptamer.
Thus, this aptamer has improved the uptake of naked mRNA by muscle cells in vivo.